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Mononucleosis, photomicrograph of cells
Mononucleosis, photomicrograph of cells
Mononucleosis - view of the throat
Mononucleosis - view of the throat


Mononucleosis spot test

Definition:

Mononucleosis spot test detects the presence of heterophile antibodies. Heterophile antibodies are antibodies that non-specifically react against different proteins and are useful in the diagnosis of infectious mononucleosis.



Alternative Names:
Monospot test; Heterophile antibody test

How the test is performed:

Blood is drawn from a vein, usually from the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic. An elastic band is placed around the upper arm to apply pressure and cause the vein to swell with blood.

A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe. During the procedure, the band is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.

In infants or young children: The area is cleansed with antiseptic and punctured with a sharp needle or a lancet. The blood may be collected in a pipette (small glass tube), on a slide, onto a test strip, or into a small container. A bandage may be applied to the puncture site if there is any bleeding.



How to prepare for the test:

No special preparation is necessary.



How the test will feel:

When the needle is inserted to draw blood, you may feel moderate pain, or only a prick or stinging sensation. You may feel throbbing at the venipuncture site for a few minutes after the blood draw.



Why the test is performed:

The mononucleosis test is done to screen for the presence of infectious mononucleosis, a disease caused by the Epstein-Barr virus (EBV). About 1 week after the onset of the disease, many patients develop heterophile antibodies. The antibodies peak at weeks 2 - 5 and may persist for several months to 1 year. A small proportion of patients with mononucleosis may never develop heterophile antibodies.




Review Date: 6/9/2005
Reviewed By: Kenneth Wener, M.D., Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, MA. Review provided by VeriMed Healthcare Network.

The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. A licensed physician should be consulted for diagnosis and treatment of any and all medical conditions. Call 911 for all medical emergencies. Links to other sites are provided for information only -- they do not constitute endorsements of those other sites. Copyright 2004 A.D.A.M., Inc. Any duplication or distribution of the information contained herein is strictly prohibited.

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