Lymph node biopsy is a test in which a lymph node or a piece of a lymph node is removed for examination under a microscope.

The lymphatic system is made up of lymph nodes connected by lymph vessels. The nodes produce white blood cells (lymphocytes) that fight infections. When an infection is present, the lymph nodes swell, produce more white blood cells, and attempt to trap the organisms that are causing the infection. The lymph nodes also try to trap cancer cells.

The test is done in an operating room in a hospital, or at an outpatient surgical facility. There are two ways the sample may be obtained:

• Needle biopsy
• Open biopsy

A needle biopsy involves inserting a needle into a node to obtain the sample. You will lie on the examination table. The biopsy site will be cleansed, and a local anesthetic will be injected. The biopsy needle is then inserted into the node. A sample is removed, pressure is applied to the site to stop the bleeding, and a bandage is applied.

An open biopsy consists of surgically removing all or part of a node. You will lie on the examination table. You may be given a sedative if you prefer. The skin over the biopsy site is cleansed, and a local anesthetic is injected (occasionally, a general anesthetic is given). A small incision is made, and the lymph node or part of the node is removed. The incision is then closed with stitches and bandaged.

The sample is then sent to the laboratory for examination.

Tell your health care provider about any drug allergies you have, which medications you are taking (including any supplements or herbal remedies), if you have bleeding problems, and if you are pregnant. You must sign a consent form.

Infants and children:

The preparation you can provide for this test depends on your child's age, previous experiences, and level of trust. For general information regarding how you can prepare your child, see the following topics:

• Infant test or procedure preparation (birth to 1 year)
• Toddler test or procedure preparation (1 to 3 years)
• Preschooler test or procedure preparation (3 to 6 years)
• Schoolage test or procedure preparation (6 to 12 years)
• Adolescent test or procedure preparation (12 to 18 years)

When the local anesthetic is injected, there will be a prick and a mild stinging. The biopsy site will be tender for a few days after the test.

The test is used to help determine the cause of lymph node enlargement (swollen glands). It may also determine whether tumors in the lymph node are cancerous or noncancerous.